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A novel PCR based DNA microanalyzer system for detection of viral genome
(University of Missouri--Columbia, 2006)
A micro-fluidic assay to quickly analyze microscopic samples of DNA is being developed for field applications. It consists of a micro-PCR chamber, micropumps, and micro-heaters. Additional components of the device include ...
Development of a FRET biosensor to detect the pathogen mycoplasma capricolum
(University of Missouri--Columbia, 2005)
Mycoplasmas are a species of bacteria that invade any type of cell and are adaptable to changing environments. We are developing an optical biosensor based on Fluorescence Resonance Energy Transfer (FRET) for detection of ...
A ratiometric fluorometer for reduced sensitivity against solvent artifacts
(University of Missouri--Columbia, 2005)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT AUTHOR'S REQUEST.] When measuring fluorescence emission intensity, both the dye concentration and the optical absorption properties of the solvent influence the measured ...
Gating of CFTR chloride channels : distinct closed states revealed by the action of AMP-PNP
(University of Missouri--Columbia, 2006)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] The CFTR chloride channel is gated by ATP through an irreversible gating cycle driven by an input of free energy of ATP hydrolysis. The open state of ...
Development of a human immunodeficiency virus (HIV-1) biosensor utilizing liquid core waveguides
(University of Missouri--Columbia, 2007)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Millions of individuals are diagnosed each year with Human Immunodeficiency Virus Type 1. Each day more individuals are receiving antiretroviral ...
Effects of oxidative stress and Alzheimer's amyloid-beta peptide on astrocytes
(University of Missouri--Columbia, 2006)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Oxidative stress and deposition of amyloid-[beta] peptide (A[beta]) in brains have been hypothesized as causes of Alzheimer's disease (AD), and research ...
Optical characterization of skeletal muscles
(University of Missouri--Columbia, 2007)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] The optical absorption and reduced scattering coefficients of bulk skeleton muscle were measured by fitting the diffusion model to spatially resolved ...
Development of a capillary based helicobacter hepaticus biosensor
(University of Missouri--Columbia, 2006)
Helicobacter hepaticus causes hepatitis in mice. Infections with H. hepaticus can invalidate animal research studies and early detection helps curtail the spread of infection. The objective of this study was to develop a ...
Nano- and micro-scale studies of exocytosis
(University of Missouri--Columbia, 2007)
Neurons and neuroendocrine cells contain vesicles packed with hormones or neurotransmitters. Upon appropriate stimulation, a rise in intracellular Ca2+ concentration triggers the fusion of vesicles with the outer membrane ...
Photoacoustic detection of metastatic melanoma in the human circulatory system
(University of Missouri--Columbia, 2006)
Detection of disseminating tumor cells can function as an early warning system, alerting the metastatic spread or recurrence of the disease. Early detection of such cells can result in preventative treatment of the disease ...
Modeling of delayed fluorescence from photosystem II and applications in assessment of drought and chemical stresses
(University of Missouri--Columbia, 2009)
Photosynthesis is not only vital for plant growth and survival but also indicative of plant-environment interactions. This research was designed to analyze photoelectron kinetics and its dependence on environmental factors. ...
Quantification of cell attachment on different materials as candidate electrodes for measurement of quantal exocytosis
(University of Missouri--Columbia, 2008)
A high throughput lab-on-a-chip device is being developed for single cell capture for the purpose of high time resolution quantal exocytosis measurement with high probability of cell docking. This device makes use of DLC ...
A fiber-optic confocal scanner for scattering tissue
(University of Missouri--Columbia, 2009)
Confocal microscopy has become an important diagnostic tool in examining scattering tissues. The high resolution of confocal microscopy and its optical sectioning capabilities lend itself as a desirable modality in examining ...
Investigative study of long-term packaging effects and donor characteristics on acellular dermis scaffolds
(University of Missouri--Columbia, 2008)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] Acellular tissue sources have become progressively popular for a variety of tissue engineering and medical applications, such as soft tissue augmentation, ...
Regulation of calcium signaling by p130PH in astrocytes
(University of Missouri--Columbia, 2009)
Ca2+ signaling is a characteristic form of astrocyte excitability, but its role in ischemia is not clearly understood. Our previous study (Ding et al., 2009) demonstrated that astrocytes exhibit enhanced Ca2+ signaling ...
Propagation of polarized light in skeletal muscle
(University of Missouri--Columbia, 2009)
Skeletal muscle is a very important tissue in animals and humans. Optical polarization imaging is a non-invasive method to study biological tissues and provides unique polarization contrast. An imaging system was developed ...
Development and applications of label-free optofluidic ring resonator biosensor
(University of Missouri--Columbia, 2009)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] This dissertation presents the development and applications of label-free optofluidic ring resonator (OFRR) sensor platform. The OFRR sensor naturally ...
Development of novel bionanocomposite scaffolds
(University of Missouri--Columbia, 2008)
[ACCESS RESTRICTED TO THE UNIVERSITY OF MISSOURI AT REQUEST OF AUTHOR.] The development of three novel bionanocomposite scaffolds is presented in this dissertation. These scaffold materials are comprised of amine-functionalized ...
Optical stimulation of quantal exocytosis on transparent microchips
(University of Missouri--Columbia, 2007)
Photorelease of caged Ca²⁺ is a uniquely powerful tool to study the dynamics of Ca²⁺-triggered exocytosis from individual cells. Using photolithography and other microfabrication techniques, we have developed transparent ...
Development of electrospun synthetic bioabsorbable fibers for a novel bionanocomposite hernia repair material
(University of Missouri--Columbia, 2008)
In order to create a more durable and biocompatible hernia repair mesh, a novel bionanocomposite material based on the crosslinking of bioabsorbable polymer fibers to decellularized porcine diaphragm tissue is proposed. ...